Withdrawal of IS 14397:1996:
The method IS 14397:1996, previously specified for the detection,
isolation, and identification of pathogenic E. coli in food,
including E. coli O157 and Vero/Shiga toxin-producing strains
under Appendix B of the FSS (Food Products Standards and Food Additives)
Regulations, 2011, has been withdrawn.
Replacement by ISO 16654:2001:
IS 14397:1996 will be replaced by ISO 16654:2001 (with
Amendments 1:2017 and 2:2023), which outlines a horizontal method for the
detection of E. coli O157 in food and animal feed.
Laboratory Accreditation Update:
As per the FSSAI order dated 20.08.2024, laboratories must update their
accredited scope to include the ISO 16654:2001 method within
six months if it has not already been incorporated during the current
accreditation cycle. IS 14397:1996 remains valid until February 2025.
Incorporation in Manual:
This updated method will be included in the forthcoming edition of the "Manual
on Methods of Analysis - Microbiological Examination of Food and Water" and
will be released shortly.
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